LARVAL EXTRACT WITH BTI STIMULATE OVIPOSITION OF Aedes sp. AND Culex sp. IN FIELD

Authors

  • Andréa Karla Lemos da Silva Sena Instituto Aggeu Magalhães
  • Maiara Santos de Menezes Instituto Aggeu Magalhães
  • Gabriel Bezerra Faierstein Instituto Aggeu Magalhães
  • Rosângela Maria Rodrigues Barbosa Instituto Aggeu Magalhães
  • Walter Soares Leal University of California Daves

Keywords:

CONTROLE DE VETORES, ECOLOGIA DE VETORES, OVIPOSIÇÃO

Abstract

The use of oviposition traps for mosquito surveillance and mosquito control is considered a sensitive and sustainable method. Traps can become more efficient when associated with biolarvicides and oviposition stimulants. Bacillus thurigiensis var. israelensis (Bti) satisfied the first criterion but finding oviposition stimulants remains a challenge. This study aimed to evaluate ovitraps and BR-OVTs associated with larval extracts of A. aegypti as an oviposition stimulant. The tests were carried out at the Aggeu Magalhães Institute in eight locations under sun and rain. The treated BR-OVTs were filled with 2 liters of larval extract (0.33 larva/ml) as an oviposition stimulant and 1g of Bti, while the controls were installed without the extract (water and Bti). Cotton fabric was used to collect eggs from Aedes sp. The rafts of Culex sp. and the eggs of Aedes sp. were accounted for and the material was renewed every two weeks. The treated ovitraps were filled with 1 liter of larval extract (0.33 larva/ml), 0.5 g of Bti, and two Eucatex blades as oviposition substrates, while controls were installed without the extract. Every fortnight, all material was renewed and eggs of the genus Aedes were accounted for. The results were analyzed by the Oviposition Activity Index (WAI) and paired T test. BR-OVTs with larval extracts collected significantly more eggs from Aedes sp. (P <0.05; WAI = +0.28) and more Culex sp. (P <0.05; WAI = +0.30) compared to controls. Evaluating the ovitraps treated with larval extracts, significantly larger collections of eggs of Aedes sp. (P <0.05; WAI = +0.38). This work demonstrates that the use of biolarvicide traps associated with oviposition stimulants potentiates the collection of mosquito eggs and can be applied on a large scale.

References

BARBOSA, R. M. R. et al. Evaluation of an oviposition-stimulating kairomone for the yellow fever mosquito, Aedes aegypti, in Recife, Brazil. Journal of vector ecology : journal of the Society for Vector Ecology, v. 35, n. 1, p. 204–7, jun. 2010.

BEN-DOV, E. Bacillus thuringiensis subsp. israelensis and Its Dipteran-Specific Toxins. p. 1222–1243, 2014.

FAIERSTEIN, G. B. et al. Conspecific and allospecific larval extracts entice mosquitoes to lay eggs and may be used in attract-and-kill control strategy. Scientific Reports, v. 9, n. 1, p. 13747, 2019.

REGIS, et al. Sustained reduction of the dengue vector population resulting from an integrated control strategy applied in two Brazilian cities. Plos One, v. 8, n. 7, p. e67682, jan. 2013.

XAVIER, M. DO N. et al. Field evaluation of sticky BR-OVT traps to collect culicids eggs and adult mosquitoes inside houses. Revista da Sociedade Brasileira de Medicina Tropical, v. 51, n. 3, p. 297–303, 2018.

Published

2019-10-20

How to Cite

Sena, A. K. L. da S., Menezes, M. S. de, Faierstein, G. B., Barbosa, R. M. R., & Leal, W. S. (2019). LARVAL EXTRACT WITH BTI STIMULATE OVIPOSITION OF Aedes sp. AND Culex sp. IN FIELD. Caderno Verde De Agroecologia E Desenvolvimento Sustentável, 9(5), b–108. Retrieved from https://gvaa.com.br/revista/index.php/CVADS/article/view/7197

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