EVALUATION OF DEVELOPMENT OF SYMBOTIC CULTURE OF BACTERIA AND YEASTS (SCOBY) IN KOMBUCHA WITH DIFFERENT SOURCES OF CAFFEINE.

Abstract

SCOBY refers to a cellulosic biofilm of yeasts and bacteria of high crystallinity, resistance to traction, insolubility, moldability, polymerization, water retention and finer fibers than as vegetable cellulose. This work aimed to develop and characterize growth and development of the floating biofilm in de Kombucha produced in different sources of caffeine: Camellia sinensis, Ilex paragauiensis, Coffea, Paullinia cupana and Theobroma cacao. The research was carried out in August 2020 at the EMBASA laboratory, in Barreiras-BA. The experiment was conducted in a completely randomized design, with five treatments (T1, green tea, T2, mate tea, T3, coffee, T4, guarana and T5, 100% cocoa) and four replications, in a set of 20 plots, carried out in triplicate , totaling 60 experimental units. The fermentation substrates were efficient for the production of a new biofilm. The pH factor did not vary with the levels of caffeine, unlike the growth of the bacterial film, which multiplied in higher levels of the substance. Treatment 5 has higher growth rates, yield, amount of dry matter, and lower moisture content compared to the others, followed by treatments 1 and 3, with median biofilm yield and approximate values ​​of moisture content 65% and 60% respectively . The treatments with the lowest performance were 2, with 80% humidity, and 4, with 75.17%. It is concluded that for the production of biofilms with the objective of obtaining biocellulose, 100% cocoa becomes the best option as an inoculum source for microbiologically active bacteria and yeasts present in Kombucha.