IDENTIFICATION OF VOLATILES ORGANIC COMPOUNDS IN SOYBEAN FLOWERS BY HS-SPME-GC-MS

Autores/as

  • José Perez da Graça Universidade Estadual de Maringá, Maringá-PR-Brasil Empresa Brasileira de Pesquisa Agropecuária, Embrapa Soja, Londrina-PR- Brasil
  • Suelen Pereira Magalhães Universidade Estadual de Londrina, Maringá-PR-Brasil Empresa Brasileira de Pesquisa Agropecuária, Embrapa Soja, Londrina-PR- Brasil
  • Hilton Cesar Rodrigues Magalhães Empresa Brasileira de Pesquisa Agropecuária, Embrapa Agroindústria Tropical, Fortaleza, CE - Brasil
  • Vagner Arnaut de Alencar de Toledo Universidade Estadual de Maringá, Maringá-PR-Brasil
  • Guilherme Julião Zocolo Empresa Brasileira de Pesquisa Agropecuária, Embrapa Agroindústria Tropical, Fortaleza, CE - Brasil
  • Décio Luiz Gazzoni Empresa Brasileira de Pesquisa Agropecuária, Embrapa Soja, Londrina-PR- Brasil
  • Clara Beatriz Hoffmann-Campo Empresa Brasileira de Pesquisa Agropecuária, Embrapa Soja, Londrina-PR- Brasil

Palabras clave:

VOLATILE COMPOUNDS, SOYBEAN, POLLINATORS, GAS CHROMATOGRAPHY

Resumen

Flowers release a variety of volatile organic compounds (VOCs) to attract pollinators. In soybean fields, bees are commonly observed foraging during the flowering, possibly attracted by visual and/or olfactory stimuli. Thus, the aim of this study was to identify the compounds produced by soybean cultivars with flowers with white and purple petals. The plants were grown in a greenhouse and the flowers collected and packed in headspace (HS) vials. For VOCs capture, the divinylbenzene/carboxene/polydimethylsiloxane SPME-fiber (DVB/CAR/PDMS) was used, and the analyses were performed on the gas chromatograph coupled to the mass spectrometer (GC-MS). In purple petal from the cultivars ‘BRS 399RR’, ‘BRS 1001IPRO’ and ‘Monsoy 6410IPRO’, 21, 39 and 35 COVs were detected, respectively. In white petals, 26 compounds were observed in ‘BRS 388 RR’, 24 in ‘DonMario 6563IPRO’ and 21 in ‘TMG 7063IPRO’. Chemometric analyses were performed and, based on PCA, PLS-DA and VIP, in all cultivars 2-hexenal; 1-hexanol; hexanal; 1-octen-3-ol; 1-hepten-3-one and furan, 2 -pentyl were detected. Multivariate statistical data showed that only 1-octen-3-ol was the compound with the highest intensity in relation to the other metabolites identified in soybean flowers. 1-hepten-one was the second compound with the largest chromatographic area, in purple petal cultivars, and 2-pentylfuran in white petal cultivars.1-Octen-3-ol was previously described in the literature as possessing stimulatory activity to Apis mellifera ligustica. Therefore, future studies of pollinator behaviour using the electroantennogram should be performed to understand the relationship of attractiveness and/or repellence to bees in response to VOCs released by soybean flowers.

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Publicado

2019-10-19

Cómo citar

Graça, J. P. da, Magalhães, S. P., Rodrigues Magalhães, H. C., Toledo, V. A. de A. de, Zocolo, G. J., Gazzoni, D. L., & Hoffmann-Campo, C. B. (2019). IDENTIFICATION OF VOLATILES ORGANIC COMPOUNDS IN SOYBEAN FLOWERS BY HS-SPME-GC-MS. Caderno Verde De Agroecologia E Desenvolvimento Sustentável, 9(5), b–52. Recuperado a partir de https://gvaa.com.br/revista/index.php/CVADS/article/view/7182